This research focused on the relationship between negative emotionality and learning from errors. Specifically, negative emotionality was expected to impair learning from errors by decreasing motivation to learn. Perceived managerial intolerance of errors was hypothesized to increase negative emotionality, whereas emotional stability was proposed to decrease negative emotionality. All the hypotheses were tested in a laboratory simulation. Contrary to the prediction, a positive association was found between negative emotionality and motivation to learn. The effects of perceived managerial intolerance of errors and emotional stability on negative emotionality were as predicted. Moreover, exploratory data analyses were conducted at the level of specific negative emotions and revealed differentiated effects of specific negative emotions on learning from errors.